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Keratin 3 (KRT3) antibody

Details for Product No. ABIN108617, Supplier: Log in to see
Antigen
  • B-CK
  • Bck
  • Ck-3
  • Ck3
  • Ckbb
  • CK3
  • K3
  • CK-3
Reactivity
Rabbit, Cow (Bovine), Human
22
3
1
1
1
Host
Mouse
19
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
17
9
3
2
2
1
1
1
1
Supplier
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Immunogen Rabbit corneal epithelial keratins
Clone AE5
Characteristics Myeloma fusion partners:
P3 x 63 Ag8 myeloma cells
Predicted molecular weight: 65 kDa.
Purification Protein G purified
Alternative Name Cytokeratin 3 (KRT3 Antibody Abstract)
Background Synonyms:
CK3 antibody,FLJ95909 antibody,K3 antibody,65 kDa cytokeratin antibody,CK 3 antibody,Cytokeratin3 antibody,Keratin 3 antibody,Keratin type II cytoskeletal 3 antibody,Keratin3 antibody,KRT 3 antibody,KRT3 antibody
UniProt Q29426
Application Notes Optimal antibody dilution should be determined by titration, however as guideline try at WB: 1/1000 - 1/3000. Flow Cyt: Use 2µg for 106 cells.
Restrictions For Research Use only
Format Liquid
Concentration 0.7 mg/mL
Buffer PBS, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
 image for anti-Keratin 3 (KRT3) antibody (ABIN108617) anti-Keratin 3 (KRT3) antibody
 image for anti-Keratin 3 (KRT3) antibody (ABIN108617) anti-Keratin 3 (KRT3) antibody (Image 2)
Product cited in: Liao, Irvine, Macewen et al.: "Development of allele-specific therapeutic siRNA in Meesmann epithelial corneal dystrophy." in: PLoS ONE, Vol. 6, Issue 12, pp. e28582, 2011 (PubMed).

Rodrigues, Ben-Zvi, Krachmer et al.: "Suprabasal expression of a 64-kilodalton keratin (no. 3) in developing human corneal epithelium." in: Differentiation; research in biological diversity, Vol. 34, Issue 1, pp. 60-7, 1987 (PubMed).

Cooper, Sun: "Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression." in: The Journal of biological chemistry, Vol. 261, Issue 10, pp. 4646-54, 1986 (PubMed).

Background publications Schermer, Galvin, Sun: "Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells." in: The Journal of cell biology, Vol. 103, Issue 1, pp. 49-62, 1986 (PubMed).