GABBR2 antibody (AA 861-912)

Catalog No. ABIN1098202

Product Details anti-GABBR2 Antibody

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Binding Specificity: AA 861-912

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABBR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-GABA (B)R2 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat GABA(B)R2. It does not cross- react with GABA(B)R1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): No cross Reactivity with GABA(B)R1

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 861-912 of rat GABA(B)R2 (accession no. NP_113990.1).

Clone: S81-2

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of ~105 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: Rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details for GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Alternative Name: GABA(B)R2 (GABBR2 Products)

Synonyms: Gpr51 antibody, GABABR2 antibody, GPR51 antibody, GPRC3B antibody, HG20 antibody, HRIHFB2099 antibody, Gb2 antibody, Gm425 antibody, GABA(B)R2 antibody, si:dkey-190l1.2 antibody, gamma-aminobutyric acid type B receptor subunit 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 S homeolog antibody, GABBR2 antibody, LOC100448699 antibody, gabbr2 antibody, Gabbr2 antibody, gabbr2.S antibody

Background: γ-aminobutyric acid type B receptor subunit 2,Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872315, PubMed:9872317, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:9872317, PubMed:10658574). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:9872315, PubMed:9872317, Ref.4, PubMed:10075644, PubMed:9872744, PubMed:10924501). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:9872315, PubMed:9872317, PubMed:10457184, PubMed:9872744, PubMed:10924501). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872317, PubMed:10457184, PubMed:9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872744, PubMed:10924501). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity). {UniProtKB:O75899, PubMed:10075644, PubMed:10457184, PubMed:10658574, PubMed:10924501, PubMed:9872315, PubMed:9872317, PubMed:9872744, Ref.4}.,GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B), and GABA(C). GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 in which GABA(B)R1 binds a ligand and GABA(B)R2 is the primary G protein contact site.,Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Perikaryon, Cell projection, dendrite,GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51

UniProt: O88871

Pathways: cAMP Metabolic Process