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Biotin antibody (FITC)

FACS, IF Host: Goat Polyclonal FITC F(ab')2 fragment

Catalog No. ABIN1100266

Datasheet as PDF

Target See all Biotin products

Biotin

Fragment

F(ab')2 fragment

Reactivity

Please inquire
Host
54

34

27
Goat
Compatible Secondaries
Clonality
72

36
Polyclonal
Conjugate
29

9

8

7

7

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to FITC
Application
55

46

44

33

31

19

14

13

12

12

9

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein Biotinylated IgG and Biotinylated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Purification

Immunoaffinity purified

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype

IgG
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo, IF (1:500 - 1:2500)

Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Restrictions

For Research Use only
Format

Liquid

Reconstitution

deionized water

Concentration

Lot specific

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freeze thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical