Hepcidin antibody (AA 22-36)

Catalog No. ABIN1101963

Product Details anti-Hepcidin Antibody

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Binding Specificity: AA 22-36

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Hepcidin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HAMP / Hepcidin.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset, Panda (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide TSGSVFPQQTGQLAE-C from the N-terminus (near) of human HAMP / Hepcidin (NP_066998.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset, Panda (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 28  kDa band in Human Liver lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.41  kDa according to NP_066998.1. The 28  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hepcidin

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: HAMP / Hepcidin (HAMP Products)

Synonyms: HEPC antibody, HFE2B antibody, LEAP1 antibody, PLTR antibody, Hepcidin antibody, Hamp1 antibody, Hepc antibody, Hepc1 antibody, HAMP antibody, hepcidin antibody, hamp1 antibody, hep1 antibody, hepcidin antimicrobial peptide antibody, hepcidin-like antibody, hepcidin-1 antibody, Ham percentage antibody, HAMP antibody, Hamp antibody, hamp antibody, LOC100135935 antibody, LOC106584587 antibody

Background: Name/Gene ID: HAMP

Synonyms: HAMP, Hepcidin antimicrobial peptide, LEAP-1, LEAP1, HEPC, Hepcidin, HFE2B, PLTR, Putative liver tumor regressor

Gene ID: 57817

NCBI Accession: NP_066998

UniProt: P81172

Pathways: Hormone Activity, Transition Metal Ion Homeostasis