LIMS2 antibody (AA 24-33)

Catalog No. ABIN1102845

Product Details anti-LIMS2 Antibody

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Binding Specificity: AA 24-33

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIMS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human LIMS2. This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Marmoset (90%), Gorilla (80%), Monkey (80%), Rabbit (80%), Opossum (80%), Turkey (80%), Chicken (80%), Platypus (80%), Nectri (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RWSNMSDALA from an internal region of human LIMS2 (NP_001129509.2, NP_001154875.1). Percent identity with other species by BLAST analysis: Human (100%), Marmoset (90%), Gorilla (80%), Monkey (80%), Rabbit (80%), Opossum (80%), Turkey (80%), Chicken (80%), Platypus (80%), Nectri (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 80kD band in Human Heart and Mouse Heart lysates after 0.5 g/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kD according to NP_001129509.2. The 80kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LIMS2

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Alternative Name: LIMS2 (LIMS2 Products)

Synonyms: PINCH2 antibody, PINCH-2 antibody, LIMS1 antibody, LIM and senescent cell antigen like domains 2 antibody, LIM zinc finger domain containing 2 antibody, Lims2 antibody, LIMS2 antibody

Background: Name/Gene ID: LIMS2

Synonyms: LIMS2, PINCH2, PINCH-2, LIM-like protein 2, ILK-binding protein

Gene ID: 55679

NCBI Accession: NP_001129509, NP_001154875

UniProt: Q7Z4I7

Pathways: Cell-Cell Junction Organization