Myc Tag (AA 410-419) antibody (Biotin)

Details for Product No. ABIN1103150, Supplier: Log in to see
Antigen
Epitope
AA 410-419
55
10
6
4
3
2
2
2
2
2
1
1
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Rabbit
161
59
17
16
3
2
1
Clonality
Polyclonal
Conjugate
Biotin
27
22
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
215
115
78
53
47
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein
Isotype IgG
Specificity This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
Purification Affinity purified
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only
Format Liquid
Reconstitution deionized water
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Supplier Images
 image for anti-Myc Tag (AA 410-419) antibody (Biotin) (ABIN1103150) anti-Myc Tag (AA 410-419) antibody (Biotin)