RIF1 antibody (AA 2432-2444)

Catalog No. ABIN1103996

Product Details anti-RIF1 Antibody

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Binding Specificity: AA 2432-2444

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human RIF1. This antibody is expected to recognize both reported isoforms (NP_060621.3, NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%) Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RNLQSRWRSPSHEN from the C-terminus of human RIF1 (NP_060621.3, NP_001171134.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%), Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 270kD band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kD according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Alternative Name: RIF1 (RIF1 Products)

Synonyms: 5730435J01Rik antibody, 6530403D07Rik antibody, AU016181 antibody, AW549474 antibody, D2Ertd145e antibody, RGD1562474 antibody, fc38c03 antibody, wu:fc38c03 antibody, si:dkey-89p3.2 antibody, replication timing regulatory factor 1 antibody, RAP1 interacting factor homolog (yeast) antibody, RIF1 antibody, Rif1 antibody, rif1 antibody

Background: Name/Gene ID: RIF1

Synonyms: RIF1

Gene ID: 55183

NCBI Accession: NP_060621, NP_001171134

UniProt: Q5UIP0

Pathways: Stem Cell Maintenance