TNFRSF1B antibody
-
- Target See all TNFRSF1B Antibodies
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This TNFRSF1B antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
- Brand
- IHC-plus™
- Specificity
- Human sTNF Receptor-II
- Purification
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human sTNF Receptor-II.
Type of Immunogen: Cell extract - Isotype
- IgG
- Top Product
- Discover our top product TNFRSF1B Primary Antibody
-
-
- Application Notes
-
Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (15 - 20 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7424 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTNF Receptor-II (0.5 μg/mL) a concentration of 15-20 μg/mL of this antibody is required. ELISA: To detect hsTNF-receptor II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor II (LS-C104278) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor II. Western Blot: To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- Alternative Name
- TNFRSF1B / TNFR2 / p75 (TNFRSF1B Products)
- Synonyms
- CD120b antibody, TBPII antibody, TNF-R-II antibody, TNF-R75 antibody, TNFBR antibody, TNFR1B antibody, TNFR2 antibody, TNFR80 antibody, p75 antibody, p75TNFR antibody, TNF-RII/TNFRSF1B antibody, TNFR-II antibody, TNFRSF1B antibody, TNF-R2 antibody, TNF-alphaR2 antibody, TNFRII antibody, TNFalpha-R2 antibody, Tnfr-1 antibody, Tnfr2 antibody, rank antibody, zgc:163064 antibody, TNF receptor superfamily member 1B antibody, tumor necrosis factor receptor superfamily, member 1b antibody, tumor necrosis factor receptor superfamily, member 1B antibody, TNFRSF1B antibody, Tnfrsf1b antibody, tnfrsf1b antibody
- Background
-
Name/Gene ID: TNFRSF1B
Family: TNF Receptor
Synonyms: TNFRSF1B, CD120b antigen, CD120b, Etanercept, p75 TNF receptor, Soluble TNFR1B variant 1, TNFBR, Tumor necrosis factor receptor, TNF-R-II, TNF-R75, TNFR2, p75TNFR, p80 TNF-alpha receptor, TBPII, TNF-R2, TNFR80, TNF-RII, TNFR-II, TNFR1B - Gene ID
- 7133
- UniProt
- P20333
- Pathways
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
-