CD103 antibody

Catalog No. ABIN1105728

Product Details anti-CD103 Antibody

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD103 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone B-ly7 recognizes an integrin containing the aE subunit which dimerizes with the b7 chain, present on hairy leukemia cells, to form the HML-1 (human mucosal lymphocyte) antigen. Clone B-ly7 is strongly reactive with hairy cell leukemia (HCL), activated monocytes, a subset of activated T and B cells (subtype of B cell chronic lymphocytic leukaemia), but not with other B cell leukemias or lymphomas. Testing by flow cytometry using a 'lyse-wash' method on a cell suspension containing HCL cells showed the following values expressed in terms of % of the total cell count: Product code: AM39027FC-N (anti-CD103 FITC) Mean % positive: 91,3 S.D.: 3,30 % CV: 3,61

Purification: Affinity chromatography

Clone: B-ly7

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: CD103 (ITGAE Products)

Synonyms: A530055J10 antibody, CD103 antibody, aM290 antibody, alpha-E1 antibody, alpha-M290 antibody, HUMINAE antibody, integrin alpha E, epithelial-associated antibody, integrin subunit alpha E antibody, Itgae antibody, ITGAE antibody

Background: The function of the CD103 integrin is related to T cell interaction with epithelium and to T cell adhesion. CD103 is expressed primarily on intra-epithelial lymphocytes and on 1-2 % of peripheral blood lymphocytes. Cellular expression: Hairy Cell Leukemia (strong), subset activated T and B cells, activated monocytes.Synonyms: HML-1 antigen, ITGAE, Integrin alpha-E, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen

Gene ID: 2209

NCBI Accession: NP_000557

UniProt: P38570