HA-Tag antibody

Details for Product No. ABIN1107475, Supplier: Log in to see
Antigen
Reactivity
Tag
450
2
1
1
1
1
1
Host
Mouse
180
177
40
34
20
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
360
201
115
108
81
70
53
40
15
13
11
7
7
5
4
4
4
4
3
2
2
1
1
1
1
1
1
Options
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Immunogen A synthetic peptide (YPYDVPDYA) from Influenza Hemagglutinin epitope coupled to KLH.
Sequence YPYDVPDYA
Clone HA-C5
Isotype IgG3
Characteristics Synonyms: Hemagglutinin Tag, HA-Tag, HA Tag
Purification Affinity Chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Notes Western blotting (1/1,000). Immunoprecipitation. Immunostaining (1/200). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1107475) anti-HA-Tag antibody
Immunofluorescence (IF) image for anti-HA-Tag antibody (ABIN1107475) anti-HA-Tag antibody (Image 2)
Background publications Hu, Luo, Ji, Chulu, Chang, Shieh, Wang, Liu: "Dual expression of the HA protein of H5N2 avian influenza virus in a baculovirus system." in: Journal of virological methods, Vol. 135, Issue 1, pp. 43-8, 2006 (PubMed).