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Vinculin antibody
VCL
Reactivity: Human, Mouse, Rat, Rabbit, Chicken
WB, IF, IHC (fro)
Host: Mouse
Monoclonal
VIN-54
unconjugated
Product Details anti-Vinculin Antibody
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Target
See all Vinculin (VCL) Antibodies
Vinculin (VCL)
Reactivity
All reactivities for Vinculin antibodies
Human, Mouse, Rat, Rabbit, Chicken
Host
All hosts for Vinculin antibodies
Mouse
Clonality
All clonalities for Vinculin antibodies
Monoclonal
Conjugate
All conjugates for Vinculin antibodies
This Vinculin antibody is un-conjugated
Application
All applications for Vinculin antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody reacts to Vinculin.
Purification
Affinity chromatography
Immunogen
Human vinculin, purified from uterus
Clone
VIN-54
Isotype
IgG1
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Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Reconstitution
Restore with 1.2 % sodium acetate or neutral PBS.
Concentration
0,1 mg/mL
Buffer
1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for Vinculin
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Target
Vinculin (VCL)
Abstract
VCL Products
Synonyms
CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody
Background
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95 % ). Vinculinis mapped to 10q22.1-q23.Synonyms: Metavinculin
Gene ID
7414
NCBI Accession
NP_003364
UniProt
P18206
Pathways
Cell-Cell Junction Organization , Maintenance of Protein Location , Signaling Events mediated by VEGFR1 and VEGFR2
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