CD1a antibody (PerCP)

Catalog No. ABIN1112083

Product Details anti-CD1a Antibody (PerCP)

Target: CD1a

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1a antibody is conjugated to PerCP

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD1a PerCP is recommended for use in flow cytometry for identification of thymocyte antigen, present thymocytes.

Clone: HI149

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Peridin-cholophyll-protein complex (Prozyme).

Sample Collection: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 20 µl of CD1a PerCP and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PP-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing Stabilizer.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD1a

Target: CD1a

Alternative Name: CD1a (CD1a Products)

Synonyms: CD1 antibody, FCB6 antibody, HTA1 antibody, R4 antibody, T6 antibody, CD1A antibody, CD1a molecule antibody, CD1 antigen antibody, T-cell surface glycoprotein CD1a antibody, CD1A antibody, CD1.1 antibody, LOC100156095 antibody, LOC101111409 antibody

Background: The human CD1 locus contains five genes, CD1A-E. CD1 molecules consist of glycosylated polypeptid alpha chains that span the cell membrane and are non-covalent associated with beta 2-microglobulin. Anti-CD1a recognizes a heavy chain molecule of 49 kDa. This antigen is expressed strongly on cortical thymocytes and on variety of antigen presenting cells including Langerhans' cells and interdigitating dendritic cells. CD1a is not expressed on early thymocytes, and is absent on mature peripheral blood T cells but intracytoplasmic expression is detected on activated T Lymphocytes.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process