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CD56 antibody (Neural Cell Adhesion Molecule 1)

Details for Product anti-NCAM1 Antibody No. ABIN111265, Supplier: Login to see
Antigen
  • NCAM1
  • ncam
  • n-cam
  • CD56
  • MSK39
  • NCAM
  • E-NCAM
  • NCAM-1
  • Ncam
  • Cd56
  • N-CAM
  • N-CAM-1
  • NCAM-C
  • NCAMC
  • fj56g09
  • ncam1
  • wu:fj56g09
Reactivity
Human, Primate
615
96
73
15
9
8
7
5
3
3
3
3
3
2
1
Host
Mouse
528
105
16
11
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD56 antibody is un-conjugated
60
53
39
27
9
8
8
8
7
6
6
6
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
350
248
124
119
97
79
77
61
27
22
7
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen KG-1 Human acute myelogenous leukaemia cell line
Clone MEM-188
Isotype IgG2a
Specificity The antibody reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with Rhesus Monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
Cross-Reactivity (Details) Species reactivity (tested):Human and non-Human Primates.
Purification Affinity Chromatography on Protein A
Alternative Name CD56 (NCAM1 Antibody Abstract)
Background CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1
Gene ID 4684
NCBI Accession NP_000606
UniProt P13591
Research Area CD Antigens, Surface Receptors of Immune Cells, Signaling
Pathways
Application Notes Western Blot. Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1/50Pretreatment: Heat retrieval (microwave oven) in citrate buffer standard ABC visualization. Positive Tissue: Positive membrane-specific reaction in adjacent peripheral nerve Stainingof paraprostatic autonomic ganglion. Staining technique: Standard ABC technique (DAB+).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 with 15 mM Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Xu, Hao, Sun et al.: "2:1 multiplexing function in a simple molecular system." in: Sensors (Basel, Switzerland), Vol. 12, Issue 4, pp. 4421-30, 2012 (PubMed).

Jakovcevski, Mo, Zecevic: "Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain." in: Neuroscience, Vol. 149, Issue 2, pp. 328-37, 2007 (PubMed).

Ohishi, Kaku, Oya et al.: "CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls." in: Gynecologic oncology, Vol. 107, Issue 1, pp. 30-8, 2007 (PubMed).

McCluggage, McKenna, McBride: "CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 26, Issue 3, pp. 322-7, 2007 (PubMed).

Yates, Rovis, Mitchell et al.: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, 2007 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Lanier, Chang, Azuma et al.: "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 12, pp. 4421-6, 1991 (PubMed).