MAP2 antibody (MAP-2) (Biotin)

Details for Product anti-MAP-2 Antibody No. ABIN111292, Supplier: Log in to see
Antigen
  • p67
  • p67eIF2
  • MAP2A
  • MAP2B
  • MAP2C
  • MAP-2
  • Mtap-2
  • Mtap2
  • MAP2R
  • xmap2
  • MGC53131
  • a730034c02
  • microtubule-associated protein 2
  • methionyl aminopeptidase 2
  • microtubule-associated protein 2-like
  • MAP2
  • METAP2
  • Map2
  • map2
  • LOC100339229
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Quail, Rat (Rattus), Xenopus laevis
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208
168
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31
18
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12
9
9
4
1
1
1
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1
Host
Mouse
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4
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP2 antibody is conjugated to Biotin
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15
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12
8
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2
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1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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100
68
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26
26
13
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6
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1
Options
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Immunogen Bovine brain microtubule associated protein 2.
Clone AP20
Isotype IgG1
Specificity Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.
Purification Protein G chromatography
Background Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker
Gene ID 4133
NCBI Accession NP_002365
UniProt P11137
Research Area Cytoskeleton
Application Notes Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
Background publications Kalcheva, Albala, Binder, Shafit-Zagardo: "Localization of specific epitopes on human microtubule-associated protein 2." in: Journal of neurochemistry, Vol. 63, Issue 6, pp. 2336-41, 1994 (PubMed).

Lewis, Ivanov, Lee, Cowan: "Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau." in: Nature, Vol. 342, Issue 6249, pp. 498-505, 1990 (PubMed).

Peng, Binder, Black: "Biochemical and immunological analyses of cytoskeletal domains of neurons." in: The Journal of cell biology, Vol. 102, Issue 1, pp. 252-62, 1986 (PubMed).

Binder, Frankfurter, Rebhun: "Differential localization of MAP-2 and tau in mammalian neurons in situ." in: Annals of the New York Academy of Sciences, Vol. 466, pp. 145-66, 1986 (PubMed).

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