Goat anti-Mouse IgG3 (Heavy Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN1118504

Product Details

Target: IgG3

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Cross-Reactivity: Mouse (Murine)

Characteristics: FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2.8

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Mouse IgG3 secondary antibody is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:200 - 1:500

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody

Background:

Synonyms: goat anti-MOUSE IgG3 Antibody fluorescein Conjugation, goat anti-MOUSE IgG3 FITC Conjugated Antibody

Background: Anti-Mouse IgG3 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.