Transferrin Receptor antibody

Catalog No. ABIN111936

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: KG-1 cells

Clone: VIP-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (= 0.2 mg/mL stock solution).

Concentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.02 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: All human source materials have tested negative for HIV1, HIV2, HBc, HCV and HBsAg antibodies. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.

Handling Advice: Avoid repeated freezing and thawing.

Storage: RT/4 °C/-20 °C

Storage Comment: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC (TFRC Products)

Synonyms: Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody

Background: CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 9606

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis