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Keratin 5 (KRT5) antibody

Details for Product No. ABIN112348
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Antigen
Synonyms CK5, DDD, DDD1, EBS2, K5, KRT5A, Tfip8, Krt2-5, AW146334, 3300001P10Rik, Sak57, ckii, wu:fb02h09, wu:fj02d02, wu:fk78b12, zfCKII, KRT75
Reactivity
Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(164), (61), (46), (29), (29), (26), (4), (3), (3), (1), (1), (1)
Host
Mouse
(104), (68), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(119), (80), (80), (48), (23), (20), (20), (20), (19), (4), (3), (1)
Pubmed 9 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN112348
423.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Cytokeratins from a human lung cancer cell line (MR21).
Clone RCK102
Isotype IgG1
Specificity RCK102 is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
Purification Purified
Alternative Name Cytokeratin 5
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Alternate names: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
Gene ID 3852
NCBI Accession NP_000415
UniProt P13647
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes RCK102 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the original vial at 2-8°C. After reconstitution store undiluted (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-Keratin 5 (KRT5) antibody RCK102 Cytokeratin staining of a human squamous cell carcinoma of the lung.
Background publications Bauwens, De Groot, Ramaekers et al.: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Broers, Carney, Klein Rot et al.: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, 1987 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Kiani, Siyal: "Dimensions of urban growth in Pakistan." in: Pakistan development review, Vol. 30, Issue 4 Pt 2, pp. 681-91, 1992 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN112348
423.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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