E-cadherin antibody

Catalog No. ABIN112362

The Mouse Monoclonal anti-E-cadherin antibody (Clone MB2) (ABIN112362) specifically detects E-cadherin in WB, FACS, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for E-cadherin antibody (ABIN112362)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MB2

Product Details anti-E-cadherin Antibody

Specificity: MB2 recognizes both the 120 kDa E-cadherin and its 80 kDa trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion.

Purification: Purified

Immunogen: MB2 is a mouse monoclonal IgG2b antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with MCF- 7/AZ cells expressing E- cadherin at their cell surface.

Isotype: IgG2b

Application Details

Application Notes: MB2 is useful for Flow cytometry, Immunoblotting, Immunocytochemistry on fixed cells(methanol fixation) and Immunohistochemistry on frozen tissues when using a PBS buffercontaining 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended dilutions: Flow cytometry 1/100-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/100-1/200.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store lyophilized antibody at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for up to two weeks. For longer storage aliquot and store frozen at -20 °C to -80 °C.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CD324 / Cadherin-1

Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin αEβ7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently down-regulated, which is consistent with its function of an invasion suppressor in normal epithelia.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

Gene ID: 999

NCBI Accession: NP_004351

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation