APOB
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Can be used to detect the existence of Apo B-48/100 in plasma and lipoproteins forImmunoassay and Immunoblot. Dilution range for Immunoblot and ELISA: 2,500-10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 1.0 mL distilled water. Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.
Concentration
1.0 mg/mL (prior to lyophilization)
Buffer
50 mM PBS, 0.1 M Sodium Chloride, pH 7.4 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute only prior to immediate use
Storage
4 °C
Storage Comment
Store the antibody undiluted for one month at 2-8 °C or (mix with an equal volume of Glycerol) at -20 °C for longer. Avoid cycles of freezing and thawing.
Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/l.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100