Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody

Antigen: Actin, alpha 2, Smooth Muscle, Aorta (ACTA2)

Synonyms: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Clonality: Monoclonal (1A4-asm-1)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rabbit, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114405

Additional Information

Characteristics: Synonyms: Alpha-actin-2, ACTVS, GIG46, Cell growth-inhibiting gene 46 protein

Alternative name: Aortic smooth muscle Actin / ACTA2

Swiss-Prot: P62736

Immunogen: N-terminal decapeptide of alpha-smooth muscle actin

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 1A4-asm-1

Description: Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells,and also in tumours derived from these cells. Actins are involved in maintaining thestructure and integrity of a cell, as well as cell motility. They are highly conserved proteinsand make up the majority of the contractile apparatus. To date, six different actin isoformshave been described.

Specificity: This antibody is specific to alpha-smooth isoform of actin. It reacts with smooth muscle cells of vessels and different parenchymes. The antibody does not cross-react with beta and gamma-cytoplasmic, alpha-sarcomericand alpha-myocardial actin isoforms. Cellular Localization: Cytoplasmic. Species: Bovine, Chicken, Human, Mouse, Rabbit and Rat. Others not tested.

Synonyms: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Application Details

Application Notes: Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: Use Actin antibodyat 1/25-1/50 dilution in an ABC method for 30 minutes at room temperature. Recommended Positive Control: Leiomyoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Extracellular Matrix, Cell/Tissue Markers

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human leiomyoma stained with Smooth Muscle Actin antibody ABIN114404/ABIN114404-05

Publications

Publications: Skalli, Schuerch, Seemayer et al.: "Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 60, Issue 2, pp. 275-85, 1989 (PubMed).

Skalli, Pelte, Peclet et al.: "Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 3, pp. 315-21, 1989 (PubMed).