TNF alpha antibody

Catalog No. ABIN114560

The Mouse Monoclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human. It has been validated for IHC (p), Func and EIA.

Quick Overview for TNF alpha antibody (ABIN114560)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: CH8820

Product Details anti-TNF alpha Antibody

Purification: Affinity Chromatography on Protein G

Immunogen: Purified recombinant Human TNF-alpha

Isotype: IgG1

Application Details

Application Notes: ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman TNF-alpha detection in combination with a Biotin-conjugated monoclonaltracer/detection antibody. Suggested capture coating dose: 1.0-2.0 g/well, Substrate: TMB. Neutralizing: This antibody neutralizes TNF-alpha activity in vitro. Use 2-5 ng of rTNF-alphaper g of MAb. Immunohistochemistry: Reacts with formalin-fixed paraffin-embedded tissues at amonoclonal antibody concentration of 10 μg/mL. Strong reaction with normal kidney, liverwith cirrhosis, and normal lung samples, positive reaction with lung cancer samples.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 9606

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome