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Endoglin (ENG) (AA 26-586) antibody

Details for Product No. ABIN115698, Supplier: Log in to see
Antigen
  • ENG
  • MGC137842
  • DKFZp469D0419
  • END
  • HHT1
  • ORW1
  • AI528660
  • AI662476
  • CD105
  • S-endoglin
  • endoglin
  • ENG
  • LOC100226246
  • Eng
Epitope
AA 26-586
26
21
15
14
14
14
13
12
12
12
10
9
9
9
8
8
7
7
7
6
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
549
213
81
34
14
9
5
5
5
3
1
1
1
Host
Rabbit
371
212
102
5
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
65
59
47
19
16
13
11
10
10
9
9
7
7
7
7
6
6
6
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
459
429
175
151
109
100
95
80
76
14
13
13
11
8
7
7
6
6
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
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Immunogen Highly pure recombinant Human soluble CD105/Endoglin produced in Insect Cells. Remarks: It consists of amino acid 26 (Glu) to 586 (Leu) and is fused to a C-terminal His-tag (6xHis).
Purification Antigen-Affinity Chromatography.
Alternative Name CD105 / Endoglin (ENG Antibody Abstract)
Background Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1
Gene ID 9606
UniProt P17813
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA: Use at 1-15 μg/mL. Western blot: Use at 1-2 μg/mL. Flow Cytometry: Use at 3-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile Water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.4 without preservatives and stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Parker, Goldring, Philip: "Endoglin is expressed on human chondrocytes and forms a heteromeric complex with betaglycan in a ligand and type II TGFbeta receptor independent manner." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 18, Issue 2, pp. 289-302, 2003 (PubMed).

Fonsatti, Del Vecchio, Altomonte, Sigalotti, Nicotra, Coral, Natali, Maio: "Endoglin: An accessory component of the TGF-beta-binding receptor-complex with diagnostic, prognostic, and bioimmunotherapeutic potential in human malignancies." in: Journal of cellular physiology, Vol. 188, Issue 1, pp. 1-7, 2001 (PubMed).

Arthur, Ure, Smith, Renforth, Wilson, Torsney, Charlton, Parums, Jowett, Marchuk, Burn, Diamond: "Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development." in: Developmental biology, Vol. 217, Issue 1, pp. 42-53, 2000 (PubMed).

Barbara, Wrana, Letarte: "Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 584-94, 1999 (PubMed).

McAllister, Grogg, Johnson, Gallione, Baldwin, Jackson, Helmbold, Markel, McKinnon, Murrell: "Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1." in: Nature genetics, Vol. 8, Issue 4, pp. 345-51, 1995 (PubMed).

Cheifetz, Bellón, Calés, Vera, Bernabeu, Massagué, Letarte: "Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19027-30, 1992 (PubMed).