IL-1 beta antibody

Catalog No. ABIN115866

The Rabbit Polyclonal anti-IL-1 beta antibody (ABIN115866) specifically detects IL-1 beta in WB, EIA and Func. The antibody is reactive with Mouse samples.

Quick Overview for IL-1 beta antibody (ABIN115866)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-1 beta Antibody

Specificity: This antibody detects Murine IL-1 beta

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-1 beta.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1 beta (10pg/mL), a concentration of 0.57-0.86 μg/mL of this antibody is required. Sandwich ELISA: To detect Mouse IL-1 beta by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Murine IL-1 beta antibody(PP006B1/PP006B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1 beta. Western Blot: To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta / IL-1B

Background: Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gene ID: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome