TNF alpha antibody

Catalog No. ABIN115973

The Rabbit Polyclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Rat. It has been validated for WB, Func and EIA.

Quick Overview for TNF alpha antibody (ABIN115973)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-TNF alpha Antibody

Specificity: This antibody detects TNF-alpha.

Purification: Affinity Chromatography employing immobilized Rat TNF-a matrix

Immunogen: Highly pure (>98%) recombinant Rat TNF-alpha

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF-alpha(2.0 ng/mL), a concentration of 0.07 - 0.1 μg/mL of this antibody is required. ELISAIndirect: To detect Rat TNF-alpha by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant Rat TNF-alpha. Sandwich: To detect Rat TNF-alpha by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, inconjunction with biotinylated Anti-Rat TNF-alpha (PP039B1 or PP039B2) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha. Western BlotTo detect Rat TNF-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat TNF-alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: TNF-alpha is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 24835

NCBI Accession: NP_036807

UniProt: P16599

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome