CCL21
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISADirect: To detect hExodus-2 by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hExodus-2. Sandwich: To detect hExodus-2 by Sandwich ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction with antiHuman Exodus-2 PP1005P1 or PP1005P2 allows the detection of at least 0.2-0.4 ng/well ofrecombinant hExodus-2. Western BlotTo detect hExodus-2 by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Human CCL21 is a beta or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups. CCL21 contains four conserved cysteine (C) residues which are characteristic of beta-chemokines. Human CCL21 cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein. Unlike other C-C chemokines, CCL21 is not chemotactic for monocytes and neutrophils. A growing body of work suggests that CCL21 influences lymphocyte homing to secondary lymphoid organs, integrin-mediated lymphocyte adhesion, and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7.Synonyms: Beta chemokine Exodus-2, CCL-21, SCYA21, SLC, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21