TNF alpha antibody (Biotin)

Catalog No. ABIN116241

The Biotin-conjugated Rabbit Polyclonal anti-TNF alpha antibody (ABIN116241) specifically detects TNF alpha in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (Biotin) (ABIN116241)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TNF alpha Antibody (Biotin)

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human TNF-Alpha (Cat.-No PA132)

Application Details

Application Notes: ElisaDirect: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hTNF-α. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL isrequired. In conjunction with polyclonal Anti-Human TNF-α as a capture antibody, it allowsthe detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α. Western Blot1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody can be stored at -20 °C. Following reconstitution, store at 2-8 °C for up to two weeks or at -20 °C for up to six months.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 9606

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome