KIT Ligand antibody
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- Target See all KIT Ligand (KITLG) Antibodies
- KIT Ligand (KITLG)
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KIT Ligand antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)
- Specificity
- Recognizes Rat SCF
- Purification
- Affinity Chromatography employing an immobilized Rat SCF matrix.
- Immunogen
- Highly pure (>98%) recombinant Rat SCF.
- Top Product
- Discover our top product KITLG Primary Antibody
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- Application Notes
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of ratSCF(15 ng/mL), a concentration of 0.08-0.12 μg/mL of this antibody is required. ELISA: To detect rat SCF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rat SCF. Western Blot: To detect rat SCF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat SCF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Target
- KIT Ligand (KITLG)
- Alternative Name
- KITLG / SCF (KITLG Products)
- Synonyms
- Xkl-1 antibody, Xsl antibody, Xsl-1 antibody, Xsl-2 antibody, steel antibody, KITLG antibody, SCF antibody, kitl antibody, kl-1 antibody, mgf antibody, scf antibody, FPH2 antibody, KL-1 antibody, Kitl antibody, MGF antibody, SF antibody, SHEP7 antibody, Clo antibody, Con antibody, Gb antibody, Kitlg antibody, Mgf antibody, SLF antibody, Sl antibody, blz antibody, contrasted antibody, 710-712 antibody, CSF antibody, KITL antibody, KIT ligand L homeolog antibody, KIT ligand antibody, kit ligand antibody, kitlg.L antibody, KITLG antibody, kitlg antibody, Kitl antibody, Kitlg antibody
- Background
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
- Gene ID
- 60427
- NCBI Accession
- NP_068615
- UniProt
- P21581
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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