IL1A antibody (Interleukin 1 alpha)

Details for Product anti-IL1A Antibody No. ABIN116863, Supplier: Log in to see
Antigen
  • Il-1
  • Il-1a
  • IL-1 alpha
  • IL-1A
  • IL1
  • IL1-ALPHA
  • IL1F1
  • IL-1alpha
  • L1A
  • interleukin 1 complex
  • interleukin 1 alpha
  • interleukin 1, alpha
  • Il1
  • Il1a
  • IL1A
Alternatives
anti-Monkey IL1A antibody for Enzyme Immunoassay
Reactivity
Human, Monkey
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102
57
11
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3
2
2
Host
Rabbit
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147
34
9
7
6
5
3
3
Clonality
Polyclonal
Conjugate
This IL1A antibody is un-conjugated
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30
16
13
9
8
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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282
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73
57
32
24
24
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22
18
12
9
8
2
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. Remarks: The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Specificity This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for Neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For Neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Purification DEAE fractionation.
Alternative Name Interleukin-1 alpha / IL-1A (IL1A Antibody Abstract)
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
Gene ID 3552
NCBI Accession NP_000566
UniProt P01583
Pathways NF-kappaB Signaling, Autophagy
Application Notes This IgG fraction antibody of anti-Human IL-1a has been tested for use in Neutralizations,ELISA, Immunohistochemistry, Flow Cytometry and Immunoblotting. It recognizes the17,000 MW mature IL-1a . Reactivity in other immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution range of 1: 100 to1: 200 is suggested for this application to detect IL-1a from supernatants or lysates of 2 x10^6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC arestimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. This producthas been assayed by Immunohistochemistry. A dilution range of 1: 200 is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry using a dilution of 1: 200 for staining of intracellular IL-1a . This
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated thawing and freezing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -20 °C.
Background publications Lonnemann, Engler-Blum, Müller, Koch, Dinarello: "Cytokines in human renal interstitial fibrosis. II. Intrinsic interleukin (IL)-1 synthesis and IL-1-dependent production of IL-6 and IL-8 by cultured kidney fibroblasts." in: Kidney international, Vol. 47, Issue 3, pp. 845-54, 1995 (PubMed).

Lonnemann, Shapiro, Engler-Blum, Müller, Koch, Dinarello: "Cytokines in human renal interstitial fibrosis. I. Interleukin-1 is a paracrine growth factor for cultured fibrosis-derived kidney fibroblasts." in: Kidney international, Vol. 47, Issue 3, pp. 837-44, 1995 (PubMed).

Cerretti, Kozlosky, Mosley, Nelson, Van Ness, Greenstreet, March, Kronheim, Druck, Cannizzaro: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, 1992 (PubMed).

Thornberry, Bull, Calaycay, Chapman, Howard, Kostura, Miller, Molineaux, Weidner, Aunins: "A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes." in: Nature, Vol. 356, Issue 6372, pp. 768-74, 1992 (PubMed).

March, Mosley, Larsen, Cerretti, Braedt, Price, Gillis, Henney, Kronheim, Grabstein: "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs." in: Nature, Vol. 315, Issue 6021, pp. 641-7, 1985 (PubMed).