Golgin B1 (GOLGB1) antibody (Atto 488)

Details for Product No. ABIN1169060, Supplier: Log in to see
Antigen
  • gcp
  • golim1
  • giantin
  • 4930428L02Rik
  • 628101
  • 6330407A06Rik
  • AU042952
  • C130074L01Rik
  • F730017E11Rik
  • Gm6840
  • mKIAA4151
  • GCP
  • GCP372
  • GOLIM1
  • golgin B1
  • golgin subfamily B member 1
  • golgi autoantigen, golgin subfamily b, macrogolgin 1
  • GOLGB1
  • golgb1
  • LOC100340420
  • Golgb1
Reactivity
Human, Mouse (Murine)
33
13
10
2
2
1
1
1
1
1
1
Host
Human
17
13
2
2
2
1
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
Atto 488
2
1
1
1
1
1
Application
Immunocytochemistry (ICC)
22
10
8
8
7
5
3
3
2
2
2
1
1
Options
Supplier
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Immunogen Human recombinant giantin.
Clone TA10
Isotype IgG2
Specificity Recognizes human and mouse giantin.
Cross-Reactivity Human, Mouse (Murine)
Purification Purified from HEK 293 cell culture supernatant.
Purity >95 % (SDS-PAGE)
Alternative Name Giantin (GOLGB1 Antibody Abstract)
Background Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
UniProt Q14789
Application Notes Optimal working dilution should be determined by the investigator.
Comment

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer In PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.
Expiry Date 12 months
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