Interleukin 33 antibody (IL33)

Details for Product anti-IL33 Antibody No. ABIN1169168, Supplier: Log in to see
Antigen
  • IL33
  • C9orf26
  • DVS27
  • IL1F11
  • NF-HEV
  • NFEHEV
  • RP11-575C20.2
  • 9230117N10Rik
  • Il-33
  • Il1f11
  • RGD1311155
  • interleukin 33
  • interleukin 33-like
  • interleukin-33-like
  • IL33
  • Il33
  • LOC100356081
  • LOC100518643
Alternatives
anti-Human Interleukin 33 antibody for Western Blotting
Reactivity
Mouse (Murine)
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Host
Mouse
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Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
This Interleukin 33 antibody is un-conjugated
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Application
ELISA, Functional Studies (Func)
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Options
Supplier
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Supplier Product No.
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Immunogen Mouse recombinant IL-33.
Clone Bondy-1-1
Isotype IgG2b
Specificity Recognizes mouse IL-33.
Cross-Reactivity Mouse (Murine)
Purification Purified from HEK 293 cell culture supernatant.
Purity >95 % (SDS-PAGE)
Endotoxin Level <0.01EU/μg purified protein (LAL test, Lonza).
Alternative Name IL-33 (IL33 Antibody Abstract)
Background Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL-33 binds to and signals through ST2 (IL-1R1) and its stimulation recruits MYD88, IRAK, IRAK4 and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14) and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases and sepsis. Anti-IL-33, mAb (recombinant) (blocking) (Bondy-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
UniProt Q8BVZ5
Pathways Production of Molecular Mediator of Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Comment

anti-IL-33 (mouse), monoclonal antibody (recombinant) (Bondy-1-1) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months
Supplier Images
ELISA image for anti-Interleukin 33 (IL33) antibody (ABIN1169168) Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (b...
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