p130 antibody (C-Term)

Catalog No. ABIN116943

This anti-p130 antibody is a Rabbit Polyclonal antibody detecting p130 in WB, IHC (p) and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for p130 antibody (C-Term) (ABIN116943)

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p130 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-p130 Antibody

Binding Specificity: C-Term

Specificity: Antiserum will specifically react with a 130 kDa Rb2 protein from Human, Rat and Mouse tissue. No reaction was observed against other related tumor supressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur.

Purification: Delipidation and Defibrination.

Immunogen: Rb2 (p130) peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Genename: RBL2

Application Details

Application Notes: Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunologicalmethods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/500-1/20,000. Western Blot: 1/500-1/2,000. Immunoprecipitation: 1/100. Immunohistochemistry on Paraffin Embedded Tissue: 1/200-1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: RBL2 / p130

Background: P130/pRb2, p107 and Rb together make up the Rb family of cell cycle regulatory proteins. As a family of proteins, the different members share several structural and functional features which include two distinct "pocket domains", interaction with viral gene products, interaction with members of the E2F family of proteins and changes in phosphorylation states leading to modulation of activity and cell cycle expression. The p130 protein is ubiquitously expressed with highest levels in terminally differentiated cells where it presumably functions to maintain the cells in a G0 state and as such has been used as a marker for G0. Depending on position within the cell cycle, p130 can exist in 4 different forms as a function of phosphorylation with Form 0 being the unphosphorylated form and the other three forms being referred to as Forms 1-3 correlating with increasing degrees of phosphorylation. Changes in levels of p130 expression have been reported in several cancers including endometrial, small cell lung, and neuroblastoma.Synonyms: 130 kDa retinoblastoma-associated protein, RB2, RBR-2, Retinoblastoma-like protein 2, Retinoblastoma-related protein 2, p130, pRb2

Gene ID: 5934

NCBI Accession: NP_005602

UniProt: Q08999

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases