MMP1 antibody (AA 98-275) (Biotin)

Catalog No. ABIN1172278

Product Details anti-MMP1 Antibody (Biotin)

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Binding Specificity: AA 98-275

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Sequence: MGHHHHHHSG SEF-AQF VLTEGNPRWE QTHLTYRIEN YTPDLPRADV DHAIEKAFQL WSNVTPLTFT KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED ERWTNNFREY NLHRVAAHEL GHSLGLSHST DIGALMYPSY TFSGDVQLAQ DDIDGIQAIY GRSQNPVQPI GPQTP

Specificity: It has been selected for its ability to recognize MMP1 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant MMP1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against MMP1 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant MMP1 (Ala98~Pro275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for MMP1

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Alternative Name: Matrix Metalloproteinase 1 (MMP1) (MMP1 Products)

Synonyms: CLG antibody, CLGN antibody, Mmp1a antibody, CG4859 antibody, Dm1-MMP antibody, Dmel\\CG4859 antibody, MMP-1 antibody, MMP1 antibody, Mmp 1 antibody, dMMP1 antibody, dm1-MMP antibody, dmmp1 antibody, l(2)k04809 antibody, mmp1 antibody, Mmp1 antibody, Mcol-A antibody, Mcola antibody, col4 antibody, mmp18 antibody, Clgn antibody, matrix metallopeptidase 1 antibody, Matrix metalloproteinase 1 antibody, matrix metalloproteinase 1 antibody, matrix metallopeptidase 1 (interstitial collagenase) antibody, matrix metallopeptidase 1a (interstitial collagenase) antibody, matrix metallopeptidase 1 S homeolog antibody, interstitial collagenase antibody, matrix metallopeptidase 8 L homeolog antibody, MMP1 antibody, Mmp1 antibody, RB11133 antibody, Mmp1a antibody, mmp1.S antibody, LOC100727966 antibody, mmp8.L antibody