MUC2 antibody (AA 5075-5179) (Biotin)
-
- Target See all MUC2 Antibodies
- MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))
-
Binding Specificity
- AA 5075-5179
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This MUC2 antibody is conjugated to Biotin
-
Application
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA
- Sequence
- MGHHHHHHSG SEF- CSTVPV TTEVSYAGCT KTVLMNHCSG SCGTFVMYSA KAQALDHSCS CCKEEKTSQR EVVLSCPNGG SLTHTYTHIE SCQCQDTVCG LPTGTSRRAR RSPRHLGSG
- Specificity
- It has been selected for its ability to recognize MUC2 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
-
Recombinant MUC2 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against MUC2 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product MUC2 Primary Antibody
-
-
- Application Notes
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
-
Content: The quality control contains recombinant MUC2 (Cys5075~Gly5179) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
-
- Target
- MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))
- Alternative Name
- Mucin 2 (MUC2) (MUC2 Products)
- Synonyms
- 2010015E03Rik antibody, MCM antibody, wnn antibody, MLP antibody, MUC-2 antibody, SMUC antibody, HH-Muc antibody, mucin 2 antibody, mucin 2, oligomeric mucus/gel-forming antibody, mucin-2 antibody, Muc2 antibody, MUC2 antibody, LOC100724045 antibody
-