CD20 antibody (Alexa Fluor 488)

Catalog No. ABIN1176883

Product Details anti-CD20 Antibody (Alexa Fluor 488)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human B lymphoma cell line

Clone: H1

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I). Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-CD20 antibody (Alexa Fluor 488) (ABIN1176883)

Nozawa, Abe, Ohno, Fukuhara, Wakasa: "Production of two monoclonal antibodies (FB1 and FB21) useful for the identification of human B lymphocytes in formalin-fixed, paraffin-embedded tissues." in: The Journal of pathology, Vol. 173, Issue 4, pp. 347-54, (1994) (PubMed).

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20 (MS4A1 Products)

Synonyms: B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody

Background: The H1(FB1) antibody reacts with a cytoplasmic domain of CD20, a 33-37-kDa tetraspan phosphoprotein that is expressed on B lymphocytes from the pre-B stage and most malignant B cells and is lost during plasma cell differentiation. Low level CD20 expression is observed on a subset of normal circulating T lymphocytes, and CD20-positive T-cell lymphomas have been reported. The CD20 molecule is associated with membrane lipid raft domains, acts as a channel for calcium ions, and is involved in regulation of B cell activation and survival. The cytoplasmic domain regions are serine and threonine rich and contain multiple phosphorylation consensus sequences. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Cytoplasmic domain