IRF7 antibody (Alexa Fluor 488)
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- Target See all IRF7 Antibodies
- IRF7 (Interferon Regulatory Factor 7 (IRF7))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This IRF7 antibody is conjugated to Alexa Fluor 488
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Application
- Intracellular Staining (ICS)
- Brand
- BD Phosflow™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human IRF-7 Recombinant Protein
- Clone
- K40-321
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product IRF7 Primary Antibody
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- Comment
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BALB/c
- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, (2003) (PubMed).
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Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, (2003) (PubMed).
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- Target
- IRF7 (Interferon Regulatory Factor 7 (IRF7))
- Alternative Name
- IRF-7 (IRF7 Products)
- Synonyms
- wu:fl24c03 antibody, zgc:66043 antibody, zgc:77947 antibody, IRF7 antibody, irf7 antibody, IRF-7H antibody, IRF7A antibody, IRF7B antibody, IRF7C antibody, IRF7H antibody, interferon regulatory factor 7 antibody, irf7 antibody, IRF7 antibody, Irf7 antibody
- Background
- Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K40-321 monoclonal antibody recognizes human IRF-7, regardless of phosphorylation status. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K40-321 does not cross-react with TBK1 by Western blot analysis using the purified antibody.
- Pathways
- TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagy
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