LAG3 antibody

Catalog No. ABIN1176990

Product Details anti-LAG3 Antibody

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse LAG3 fusion protein

Clone: C9B7W

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-LAG3 antibody (ABIN1176990)

Huang, Workman, Flies, Pan, Marson, Zhou, Hipkiss, Ravi, Kowalski, Levitsky, Powell, Pardoll, Drake, Vignali: "Role of LAG-3 in regulatory T cells." in: Immunity, Vol. 21, Issue 4, pp. 503-13, (2004) (PubMed).

Workman, Rice, Dugger, Kurschner, Vignali: "Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3)." in: European journal of immunology, Vol. 32, Issue 8, pp. 2255-63, (2002) (PubMed).

Huard, Mastrangeli, Prigent, Bruniquel, Donini, El-Tayar, Maigret, Dréano, Triebel: "Characterization of the major histocompatibility complex class II binding site on LAG-3 protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5744-9, (1997) (PubMed).

Miyazaki, Dierich, Benoist, Mathis: "Independent modes of natural killing distinguished in mice lacking Lag3." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 405-8, (1996) (PubMed).

Baixeras, Huard, Miossec, Jitsukawa, Martin, Hercend, Auffray, Triebel, Piatier-Tonneau: "Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens." in: The Journal of experimental medicine, Vol. 176, Issue 2, pp. 327-37, (1992) (PubMed).

Triebel, Jitsukawa, Baixeras, Roman-Roman, Genevee, Viegas-Pequignot, Hercend: "LAG-3, a novel lymphocyte activation gene closely related to CD4." in: The Journal of experimental medicine, Vol. 171, Issue 5, pp. 1393-405, (1990) (PubMed).

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: CD223 (LAG3 Products)

Synonyms: CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody

Background: The C9B7W antibody reacts with an epitope in the D2 domain of CD223 (LAG3), the 70-kDa protein encoded by Lymphocyte-activation gene 3 (Lag3). A fusion protein consisting of the entire extracellular region of mouse LAG3 with mouse IgG1 was used as immunogen. CD223 is a type-I membrane protein with four extracellular Ig-like domains, it is structurally homologous to CD4, and, like CD4, it binds MHC class II molecules. However, unlike CD4, it is not expressed on resting T lymphocytes, in both the human and the mouse. In the mouse, as previously described in the human, CD223 expression is upregulated on T lymphocytes (both CD4+ and CD8+) activated through the T-cell receptor (TCR) and on IL-2-activated NK (LAK) cells, and it is not detected on B cells, dendritic cells, or Phorbol 12-myristate 13-acetate (PMA)-stimulated splenocytes. Studies on human peripheral T lymphocytes suggest that CD223 associates with the TCR to downregulate TCR signaling. In contrast, in vivo and in vitro evaluations of vaccination protocols in mice suggest that CD223 promotes immune responses by activating antigen-presenting cells. Furthermore, NK cells of Lag3-/- mice display defects in their capacity to kill certain tumor cells. Mouse CD223 also has been demonstrated to contribute to the suppressor function of T regulatory cells and the C9B7W antibody has been shown to inhibit this function in vitro and in vivo. Therefore, CD223 appears to play complex roles in the regulation of immune responses. Although the C9B7W antibody is unable to block the binding of MHC class II-IgG2a fusion protein to CD223, it is able to block the CD223-mediated inhibition of IL-2 production by a T-cell hybridoma responding to antigen.
Synonyms: LAG3

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints