Western Blotting (WB), ELISA, Neutralization (Neut)
Brand
BD Pharmingen™
Characteristics
Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-8. Neutralization activity may be measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells. Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization. 50% Neutralization (ND50) at 8 - 10 myg/mL >95% Neutralization at 150 - 500 myg/mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility
0.2 μm filtered
Endotoxin Level
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
IL8
Reactivity: Human
WB, ELISA, IHC (p), IF
Host: Rabbit
Polyclonal
unconjugated
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative
Azide free
Storage
4 °C
Storage Comment
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).
The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form). This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines: Human IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- gamma, MCP-1, MCP-2, MCP-3, MIP-1alpha, MIP-1beta, NAP-2, PF4, RANTES, SCF, TNF, LT-alpha.