MDC1 antibody (AA 679-694)

Catalog No. ABIN117918

Product Details anti-MDC1 Antibody

Target: MDC1 (Mdc1 (MDC1))

Binding Specificity: AA 679-694

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: Peptide Sequence: G-G-T-K-D-S-E-D-N-Y-G-D-S-E-D-L

Specificity: This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 679-694 of Human MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 is hyper-phosphorylated in an ATM- dependent manner.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed by ELISA against 0.1 μg of the immunizing peptide. A1: 3,000 to 1: 12,000 dilution of the antibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1 (MDC1 Products)

Synonyms: 6820401C03 antibody, AA413496 antibody, Nfbd1 antibody, mKIAA0170 antibody, NFBD1 antibody, mediator of DNA damage checkpoint 1 antibody, mediator of DNA-damage checkpoint 1 antibody, Mdc1 antibody, MDC1 antibody

Background: MDC1 (Mediator of DNA damage checkpoint protein 1) plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, both within S phase and G2/M. It is thought to act as a scaffold protein during the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139. MDC1 is also involved in downstream events subsequent to the recruitment of these proteins. MDC1 has tandem repeat BRCT domains that are required for localisation to chromatin that flanks sites marked by S139 phosphorylated histone H2A.X. MDC1 is phosphorylated following exposure to ionising radiation (IR), ultraviolent radiation (UV), and hydroxyurea (HU). MDC1 is also phosphorylated during G2/M and during activation of the mitotic spindle checkpoint.Synonyms: KIAA0170, MDC1, Mediator of DNA damage checkpoint protein 1, NFBD1

Gene ID: 9656

NCBI Accession: NP_055456

UniProt: Q14676