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p65 antibody (Nuclear Factor-KB P65) (N-Term)

Details for Product anti-NFkBP65 Antibody No. ABIN117926, Supplier: Log in to see
Antigen
  • NFkB
  • NFKB3
  • p65
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
N-Term, NLS Domain
135
74
68
57
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48
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35
31
26
26
22
16
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2
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2
2
2
1
1
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1
1
1
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1
1
1
1
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Reactivity
Human
1187
588
450
45
36
28
26
13
10
4
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
1043
138
15
10
7
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
35
35
27
21
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12
10
10
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10
10
6
6
4
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1
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1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
841
471
369
223
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135
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90
71
30
18
17
11
8
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4
2
2
1
1
Supplier
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkappaB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Sequence Peptide Sequence: R-I-E-E-K-R-K-R-T-Y-E-T
Specificity This affinity purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFκB heterodimer. Therefore, the antibody selectively binds to the activated form of NFκB. Anti-NFκB p65 NLS may react non-specifically with other proteins.
Purification Affinity Purified
Alternative Name RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
Background NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. IκB- α binding masks the NFκB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFκB and set off signalling cascades that ultimately converge on the IκB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IκB-α and this phophorylation event targets IκB-α for degradation. As a consequence, NFκB NLS is uncovered and nuclear translocation occurs.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
Gene ID 5970
NCBI Accession NP_001138610
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Suitable for Immunohistochemistry, Immunoblotting, ELISA and supershift assays. Thisproduct was assayed by Immunoblot and found to be reactive against NFκB p65 NLS at adilution of 1: 2,000 followed by reaction with Peroxidase conjugated Affinity Purified
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p65 antibody (Nuclear Factor-KB P65) (N-Term) (ABIN117926) Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 ...
Immunofluorescence (IF) image for anti-p65 antibody (Nuclear Factor-KB P65) (N-Term) (ABIN117926) Immunofluorescencechemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to dete...
Background publications Zhang, Ghosh: "Molecular mechanisms of NF-kappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors." in: Journal of endotoxin research, Vol. 6, Issue 6, pp. 453-7, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

Delfino, Walker: "Hormonal regulation of the NF-kappaB signaling pathway." in: Molecular and cellular endocrinology, Vol. 157, Issue 1-2, pp. 1-9, 2000 (PubMed).

Unlap, Jope: "Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 83-9, 1997 (PubMed).

Zabel, Henkel, Silva, Baeuerle: "Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus." in: The EMBO journal, Vol. 12, Issue 1, pp. 201-11, 1993 (PubMed).

Lenardo, Baltimore: "NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control." in: Cell, Vol. 58, Issue 2, pp. 227-9, 1989 (PubMed).

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