TLK1 antibody (Isoform 1, pSer695)

Catalog No. ABIN117936

Product Details anti-TLK1 Antibody

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Binding Specificity: AA 737-751, Isoform 1, pSer695

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLK1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Sequence: Human Tlk-1 (isoform 1), 766 aa, predicted MW 86.7 kDa: 1 msvqsssgsl egppswsqls tsptpgsaaa arsllnhtpp sgrpregamd elhsldprrq 61 ellearftgv asgstgstgs csvgakastn nessnhsfgs lgslsdkese tpekkqsess 121 rgrkrkaenq nessqgksig grghkisdyf eyqggngssp vrgippairs pqnshshstp 181 sssvrpnsps ptalafgdhp ivqpkqlsfk iiqtdltmlk laalesnkiq dlekkegrid 241 dllrancdlr rqideqqkll ekykerlnkc ismskkllie kstqeklssr eksmqdrlrl 301 ghfttvrhga sfteqwtdgf afqnlvkqqe wvnqqredie rqrkllakrk pptannsqap 361 stnsepkqrk nkavngaend pfvrpnlpql ltlaeyheqe eifklrlghl kkeeaeiqae 421 lerlervrnl hirelkrinn ednsqfkdhp tlnerylllh llgrggfsev ykafdlyeqr 481 yaavkihqln kswrdekken yhkhacreyr ihkeldhpri vklydyfsld tdtfctvley 541 cegndldfyl kqhklmseke arsivmqivn alrylneikp piihydlkpg nillvdgtac 601 geikitdfgl skimdddsyg vdgmdltsqg agtywylppe cfvvgkeppk isnkvdvwsv 661 gviffqclyg rkpfghnqsq qdilqentil katevqfpvk pvvsseakaf irrclayrke 721 drfdvhqlan dpyllphmrr snssgnlhma gltasptpps ssiity

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 737-751 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) and byWestern blot (1: 200- 1: 2,000). Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 byWestern blotting in the appropriate cell lysate or extract. This antibody reacts with bothphosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) ofthe protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: TLK1 (TLK1 Products)

Synonyms: PKU-beta antibody, 4930545J15Rik antibody, fe11e12 antibody, si:dkey-251e16.4 antibody, tlk1 antibody, wu:fe11e12 antibody, TLK1 antibody, fb76h12 antibody, si:dkey-88m1.1 antibody, wu:fb76h12 antibody, DKFZp459L163 antibody, tousled like kinase 1 antibody, tousled-like kinase 1 antibody, tousled-like kinase 1b antibody, tousled like kinase 1 like antibody, serine/threonine-protein kinase TOUSLED antibody, tousled-like kinase 1a antibody, Serine/threonine-protein kinase tousled-like 1 antibody, TLK1 antibody, Tlk1 antibody, tlk1b antibody, TLK1L antibody, LOC542181 antibody, tlk1a antibody, tlk1 antibody, tlk-1 antibody

Background: Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent. Tlk-1 heterodimerizes with Tlk-2. Multiple isoforms of the protein have been detected. Mature Tlk-1 is reported to have an apparent molecular weight of ~86 kDa, ~89 kDa and ~64 kDa corresponding to isoforms 1, 2 and 3 respectively.Synonyms: KIAA0137, PKU-beta, Serine/threonine-protein kinase tousled-like 1, Tousled-like kinase 1

NCBI Accession: NP_036422

UniProt: Q9UKI8