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Details for Product No. ABIN117942

Anaphase Promoting Complex Subunit 2 (ANAPC2) (C-Term), (AA 810-822) antibody

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Antigen
Synonyms
APC2, RP11-350O14.5, 9230107K09Rik, AL024279, Apc2, Emi4, Imi4, mKIAA1406, F28I8.30, F28I8_30, anaphase-promoting complex/cyclosome 2, anapc2, ANAPC2, DDBDRAFT_0203816, DDBDRAFT_0234264, DDB_0203816,  ... show more
Epitope
»Alternatives C-Term, AA 810-822
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN117942
Quantity 0.1 mL
Price
379.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C- terminal) coupled to KLH.
Sequence Human APC2 (822 aa 93.8 kDa): 1 maaavvvaeg dsdsrpgqel lvawntvstg lvppaalglv ssrtsgavpp keeelraave 61 vlrghglhsv leewfvevlq ndlqanispe fwnaisqcen sadepqclll lldafglles 121 rldpylrsle llekwtrlgl lmgtgaqglr eevhtmlrgv lffstprtfq emiqrlygcf 181 lrvymqskrk geggtdpele geldsryarr ryyrllqspl cagcssdkqq cwcrqaleqf 241 hqlsqvlhrl sllervsaea vtttlhqvtr ermedrcrge yersflrefh kwiervvgwl 301 gkvflqdgpa rpaspeagnt lrrwrchvqr ffyriyaslr ieelfsivrd fpdsrpaied 361 lkyclertdq rqqllvslka aletrllhpg vntcdiitly isaikalrvl dpsmvileva 421 cepirrylrt redtvrqiva gltgdsdgtg dlavelsktd pasletgqds eddsgepedw 481 vpdpvdadpg kssskrrssd iisllvsiyg skdlfineyr slladrllhq fsfspereir 541 nvellklrfg eapmhfcevm lkdmadsrri nanireedek rpaeeqppfg vyavilssef 601 wppfkdekle vpediraale ayckkyeqlk amrtlswkht lglvtmdvel adrtlsvavt 661 pvqavillyf qdqaswtlee lskavkmpva llrrrmsvwl qqgvlreepp gtfsvieeer 721 pqdrdnmvli dsddesdsgm asqadqkeee lllfwtyiqa mltnleslsl driynmlrmf 781 vvtgpalaei dlqelqgylq kkvrdqqlvy sagvyrlpkn cs
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human APC2. Cross reactivity may also occur with APC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
Purification Delipidation and defibrination.
Alternative Name APC2 / ANAPC2
Background APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase- anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.
Alternate names: Anaphase-promoting complex subunit 2, Cyclosome subunit 2, KIAA1406
Gene ID 29882
NCBI Accession NP_037498.1
UniProt Q9UJX6
Research Area Coagulation
Application Notes Western blot (1: 500-1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others. Coimmunoprecipitation of related proteins (APC11) does occur. A 93.8 kDa bandcorresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 canbe used as a positive control. ELISA (1: 2,000-1: 10,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Zachariae, Shevchenko, Andrews et al.: "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1216-9, 1998 (PubMed).

Yu, Peters, King et al.: "Identification of a cullin homology region in a subunit of the anaphase-promoting complex." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1219-22, 1998 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Tang, Li, Bharadwaj et al.: "APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex." in: Molecular biology of the cell, Vol. 12, Issue 12, pp. 3839-51, 2001 (PubMed).

Wang, Yu, Xiang et al.: "Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride." in: FEMS microbiology letters, Vol. 279, Issue 2, pp. 217-25, 2008 (PubMed).

Alternatives for antigen "Anaphase Promoting Complex Subunit 2 (ANAPC2)", type "Antibodies"
Hosts (15), (12)
Reactivities (27), (6), (4), (1)
Applications (26), (8), (8), (8), (5), (3), (3), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1), (1), (1)
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