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TLR4 antibody (Toll-Like Receptor 4) (FITC)

Details for Product anti-TLR4 Antibody No. ABIN119329, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll-like receptor 4
  • TLR4
  • Tlr4
Alternatives
anti-Dog (Canine) TLR4 antibody for Flow Cytometry
Reactivity
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
502
247
141
74
50
49
42
26
12
10
4
2
2
1
1
Host
Mouse
284
253
55
10
Clonality (Clone)
Monoclonal ()
Conjugate
This TLR4 antibody is conjugated to FITC
48
43
40
16
13
12
12
8
8
8
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7
6
6
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6
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5
4
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4
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3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
370
295
134
132
124
108
96
96
34
27
26
24
21
16
12
8
8
4
4
3
3
2
2
1
1
1
1
Options
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Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Specificity This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Purification Affinity Chromatography on Protein G.
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
Gene ID 7099, 9606
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin
Application Notes Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 l whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-TLR4 antibody (Toll-Like Receptor 4)  (FITC) (ABIN119329) Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC (ABIN1...
Background publications Jiang, Akashi, Miyake, Petty: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).