Flow cytometry: Use 10 μL of neat - 1/5 diluted antibody solution to label 10^6 cells or 100 μL whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water.
Buffer
PBS, pH 7.4, containing 1 % BSA and 0.09 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
Do Not Use Sodium Azide as Preservative.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Avanzi, Leoni, Rotola, Alviano, Solimando, Lanzoni, Bonsi, Di Luca, Marchionni, Alvisi, Ripalti: "Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection." in: PLoS ONE, Vol. 8, Issue 8, pp. e71412, (2013) (PubMed).
CD21 is a 145 kD glycoprotein expressed by mature B cells and follicular dendritic cells. CD21 acts as a receptor for complement components C3d, C3dg and iC3b and also for Epstein Barr Virus.Synonyms: C3DR, C3d receptor, CR2, Complement C3d receptor, Complement receptor type 2, Dendritic Cell Marker, EBV Receptor, Epstein-Barr virus receptor