HLA-DR antibody

Catalog No. ABIN120140

The Rat Monoclonal anti-HLA-DR antibody is suitable to detect HLA-DR in samples from Human and Mouse. It has been validated for FACS, IHC (p) and IHC (fro).

Quick Overview for HLA-DR antibody (ABIN120140)

Target: HLA-DR

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YE2-36-HLK

Product Details anti-HLA-DR Antibody

Purification: Affinity chromatography on Protein G

Immunogen: EHR-B Ramos cells. Spleen cells from immunised AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysineparaformaldehyde) fixation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA Class II DR

Background: HLA DR is expressed by B lymphocytes, activated T cells and by monocytes.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response