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HLA-DR antibody (HLA-DR) Primary Antibody

HLA-DR Reactivity: Human FACS, IHC, ISt, StM, WB Host: Mouse Monoclonal TAL 1B5
Catalog No. ABIN6939639
$593.17
Plus shipping costs $45.00
100 μg ABIN6939639
100 μg ABIN6939639
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  • Target
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This HLA-DR antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM), Western Blotting (WB)
    Purification
    Purified by Protein A/G
    Clone
    TAL 1B5
    Isotype
    IgG1, kappa
  • Application Notes

    Positive Control: Raji, Ramos or Daudi whole cell lysates. Spleen, tonsil or liver tissue lysates. Human melanoma, tonsil or lymph node.

    Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    HLA-DR
    Background
    Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain ( -Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
    Molecular Weight
    36kDa (alpha chain), 27kDa (beta chain), 63kDa (HLA-DR dimer)
    Gene ID
    3122
    UniProt
    P01903
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