Integrin beta 2 antibody (FITC)

Catalog No. ABIN120419

Product Details anti-Integrin beta 2 Antibody (FITC)

Target: Integrin beta 2 (ITGB2)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. The antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Purification: Affinity chromatography on Protein A

Immunogen: FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Clone: WT-3

Isotype: IgG1

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2 (ITGB2 Products)

Background: CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 25021

NCBI Accession: NP_036843

UniProt: Q9JI30

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation