Transferrin Receptor (p90, CD71) (TFRC) antibody

Details for Product No. ABIN120509
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms trfr, CD71, T9, TFR, TFR1, TR, TRFR, p90, TFRC, tfr, cd71, tfr1, LOC100219280
Reactivity
Rat (Rattus)
(169), (99), (18), (11), (1), (1), (1)
Host
Mouse
(144), (76), (36), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(38), (28), (26), (8), (5), (2), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunoelectron Microscopy (IEM), Flow Cytometry (FACS)
(184), (56), (52), (48), (29), (26), (14), (12), (11), (8), (4), (3), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
Catalog no. ABIN120509
Quantity 1 mg
Price
720.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen PHA activated rat lymphocytes. Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
Clone OX-26
Isotype IgG2a
Specificity This antibody recognises CD71 (Transferrin Receptor). This antibody also binds to a number of non-dividing normal tissues. The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage.
Purification Affinity Chromatography on Protein G.
Alternative Name CD71 / TFRC
Background CD71 is a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimers, Parkinsons, Huntingtons and Multiple Sclerosis.
Alternate names: TfR1, Transferrin receptor protein 1, p90
NCBI Accession 10116
UniProt Q99376
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10ul of 1/50-1/200 diluted antibody to label 10e6 cells in 100 ul. Western Blot: This antibody detects a band of approximately 95kDa in ConA cell lysates under Reducing conditions (MWT 195kDA without reduction). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Clone MRC OX-26 is reported as suitable for use in Electron Microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody Staining of rat spleen lymphocytes with MOUSE ANTI RAT CD71 (ABIN120509).
Background publications Jefferies, Brandon, Williams et al.: "Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor." in: Immunology, Vol. 54, Issue 2, pp. 333-41, 1985 (PubMed).

Jefferies, Brandon, Hunt et al.: "Transferrin receptor on endothelium of brain capillaries." in: Nature, Vol. 312, Issue 5990, pp. 162-3, 1984 (PubMed).

Yefimova, Jeanny, Keller et al.: "Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons rats." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 537-45, 2002 (PubMed).

Lipardi, Ruggiano, Perrone et al.: "Differential recognition of a tyrosine-dependent signal in the basolateral and endocytic pathways of thyroid epithelial cells." in: Endocrinology, Vol. 143, Issue 4, pp. 1291-301, 2002 (PubMed).

General Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Gambling, Danzeisen, Gair et al.: "Effect of iron deficiency on placental transfer of iron and expression of iron transport proteins in vivo and in vitro." in: The Biochemical journal, Vol. 356, Issue Pt 3, pp. 883-9, 2001 (PubMed).

Hosts (144), (76), (36), (1)
Reactivities (169), (99), (18), (11), (1), (1), (1)
Applications (184), (56), (52), (48), (29), (26), (14), (12), (11), (8), (4), (3), (1), (1), (1), (1), (1), (1)
Conjugates (38), (28), (26), (8), (5), (2), (2), (1), (1), (1), (1)
Epitopes (7), (7), (4), (3), (3), (2), (2), (1), (1)
back to top