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HtrA Serine Peptidase 2 (HTRA2) (AA 134-458) antibody

Details for Product No. ABIN121159
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Antigen
Synonyms CG8464, DmHtrA2, Dmel\\CG8464, HtrA2/Omi, Omi, dOmi, domi, htrA2, omi, HTRA2, PRSS25, OMI, PARK13, AI481710, Prss25, mnd2
Epitope
AA 134-458
(20), (12), (7), (7), (6), (6), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(123), (38), (37), (13), (13), (1)
Host
Mouse
(86), (37)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(93), (64), (41), (22), (10), (9), (6), (3), (3), (2), (2), (2)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN121159
412.50 $
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Immunogen Recombinant human HtrA2/Omi (134-458 aa) purified from E. coli
Clone 1B3
Isotype IgG1
Purification Protein-G affinity chromatography
Alternative Name HTRA2 / PRSS25
Background HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Recently, HtrA2 has been known to contribute both to caspase-dependent and caspase-independent cell death. Alternate Names: PARK13, EC 3.4.21.108, Serine protease HTRA2 (mitochondrial), High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25.
UniProt O43464
Application Notes ELISA. Western blot (1:500 - 1:2,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-HtrA Serine Peptidase 2 (HTRA2) (AA 134-458) antibody Western blot analysis.,.The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
General Zumbrunn, Trueb: "Primary structure of a putative serine protease specific for IGF-binding proteins." in: FEBS letters, Vol. 398, Issue 2-3, pp. 187-92, 1997 (PubMed).

Validation Images
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