DIABLO antibody (AA 225-239)

Catalog No. ABIN121595

Product Details anti-DIABLO Antibody

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Binding Specificity: AA 225-239

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 225-239 of human Smac/DIABLO

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 μg/mL. Immunohistochemistry on paraffin sections: 5 μg/mL, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Ovary tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO / SMAC (DIABLO Products)

Synonyms: smac antibody, smac3 antibody, diablo-s antibody, 0610041G12Rik antibody, 1700006L01Rik antibody, AU040403 antibody, Smac antibody, DFNA64 antibody, SMAC antibody, DBO antibody, XSmac antibody, diablo, IAP-binding mitochondrial protein antibody, diablo IAP-binding mitochondrial protein antibody, diablo, IAP-binding mitochondrial protein L homeolog antibody, diablo antibody, Diablo antibody, DIABLO antibody, diablo.L antibody

Background: Inhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X-linked inhibitor of apoptosis (XIAP) is a member of the IAP family. Smac/DIABLO is expressed in a variety of tissues and has been identified as a negative regulator of XIAP.Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity