GADD34 antibody (C-Term)

Catalog No. ABIN121667

The Goat Polyclonal anti-GADD34 antibody is suitable to detect GADD34 in samples from Human. It has been validated for WB.

Quick Overview for GADD34 antibody (C-Term) (ABIN121667)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GADD34 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GADD34 Antibody

Binding Specificity: C-Term

Purification: Affinity chromatography

Immunogen: Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145)

Application Details

Application Notes: Western Blot: 0.5 μg/mL - 2 μg/mL, this antibody detects a band of approximately 80-90 kDa in U937 cell lysates, an overnight incubation time of 12 hours is recommended for this antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: TRIS buffered saline pH 7.3 containing 0.02 % Sodium Azide and 0.5 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for GADD34

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: GADD34 / PPP1R15A

Background: GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress. Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status. Reports suggest that GADD34 may be involved in signal transuction pathway controlling cell cycle arrest and/or apoptosis following DNA damage.Synonyms: Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, Protein phosphatase 1 regulatory subunit 15A

Pathways: ER-Nucleus Signaling, Unfolded Protein Response